تشخیص سلول های سرطانی در جریان خون، به عنوان یک نشان گر زیستی ارزشمند کم تهاجمی انقلابی در مدیریت سرطان ایجاد کرده است. این مقاله به مرور جامع کاربردهای هوش مصنوعی شامل یادگیری ماشین و یادگیری عمیق در شناسایی و شمارش خودکار سلول های سرطانی در جریان خون از نمونه های خونی واقعی می پردازد. با بررسی سیستماتیک مطالعات پیشین، مزایا مانند سرعت و دقت بالا و معایب مانند وابستگی شدید به حجم و کیفیت داده های آموزشی در هر روش تحلیل شده است. یافته ها نشان می دهند که عملکرد مدل های هوش مصنوعی به طور قابل توجهی به کیفیت تصاویر وابسته است و همچنین فرآیند آموزش آنها اغلب زمان بر می باشد. برای غلبه بر این چالش، راهکارهایی مانند پیش پردازش داده، تکنیک های تقویت مصنوعی داده و استفاده از حلقه های بازخوردی پیشنهاد می شوند. با وجود نتایج امیدوار کننده ای که از پژوهش های انجام شده پیشین بدست آمده است برای کاربردی کردن این روش در محیط های بالینی واقعی، نیاز مبرمی به توسعه الگوریتم های عمومی تر، قوی تر و کمتر وابسته به داده های حجیم به عنوان نیاز اصلی این حوزه تحقیقاتی باقی است.
[1]R. Lawrence, M. Watters, C. R. Davies, K. Pantel, and Y.-J. Lu, "Circulating tumour cells for early detection of clinically relevant cancer," Nature Reviews Clinical Oncology, vol. 20, no. 7, pp. 487-500, 2023,https://doi.org/10.1038/s41571-023.
[2]H. Yao, L. Wen, Z. Li, and C. Xia, "Analysis of diagnostic value of CTC and CTDNA in early lung cancer," Cellular and Molecular Biology, vol. 69, no. 6, pp. 57-62, 2023,https://doi.org/10.14715/cmb.
[3]A. Strati, A. Markou, E. Kyriakopoulou, and E. Lianidou, "Detection and molecular characterization of circulating tumour cells: challenges for the clinical setting," Cancers, vol. 15, no. 7, p. 2185, 2023,https://doi.org/10.3390/cancers15072185.
[4]M. Vidlarova et al., "Recent advances in methods for circulating tumor cell detection," International Journal of Molecular Sciences, vol. 24, no. 4, p. 3902, 2023,https://doi.org/10.3390/ijms24043902.
[5]Galanzha, E.I.; Zharov, V.P. Circulating Tumor Cell Detection and Capture by Photoacoustic Flow Cytometry in Vivo and ex Vivo. Cancers, 5, 1691-1738, 2013, https://doi.org/10.3390/cancers5041691.
[6]T. N. A. Nguyen, P.-S. Huang, P.-Y. Chu, C.-H. Hsieh, and M.-H. Wu, "Recent progress in enhanced cancer diagnosis, prognosis, and monitoring using a combined analysis of the number of circulating tumor cells (CTCs) and other clinical parameters," Cancers, vol. 15, no. 22, p. 5372, 2023,https://doi.org/10.3390/cancers15225372.
[7]M. Li, J. Shi, Y. Zhang, S. Cui, L. Zhang, and Q. Shen, "ECL cytosensor for sensitive and label-free detection of circulating tumor cells based on hierarchical flower-like gold microstructures," Analytica Chimica Acta, vol. 1303, p. 342505, 2024, https://doi.org/10.1016/j.aca.2024.342505.
[8]J. Bialek, A. Muthe, S. Yankulov, F. Kawan, G. Gakis, and G. Theil, "Optimizing CTC isolation techniques for molecular characterization of circulating tumor cells in clear cell renal cell carcinoma: A comparative study of EpCAM-based and density-based methods," Cancer Research, vol. 84, no. 6_Supplement, pp. 3689-3689, 2024,https://doi.org/10.1158/1538-7445.AM2024-3689.
[9] X. Ye et al., "An adhesion-based method for rapid and low-cost isolation of circulating tumor cells," Clinica Chimica Acta, vol. 547, p. 117421, 2023,https://doi.org/10.1016/j.cca.2023.117421.
[10] Y. Zhang, Z. Zhang, D. Zheng, T. Huang, Q. Fu, and Y. Liu, "Label-free separation of circulating tumor cells and clusters by alternating frequency acoustic field in a microfluidic chip," International Journal of Molecular Sciences, vol. 24, no. 4, p. 3338, 2023,https://doi.org/10.1016/j.cca.2023.117421.
[11] Y. Li et al., "Label-free detection and simultaneous viability determination of CTCs by lens-free imaging cytometry," Analytical and Bioanalytical Chemistry, pp. 1-13, 2024,DOI: 10.1007/s00216-024-05624-y.
[12] S. Zhu et al., "Liquid Biopsy Instrument for Ultra-Fast and Label-Free Detection of Circulating Tumor Cells," Research, vol. 7, p. 0431, 2024,https://doi.org/10.34133/research.0431.
[13] M. J. Poellmann et al., "Nanotechnology and machine learning enable circulating tumor cells as a reliable biomarker for radiotherapy responses of gastrointestinal cancer patients," Biosensors and Bioelectronics, vol. 226, p. 115117, 2023,https://doi.org/10.1016/j.bios.2023.115117.
[14] K. Pastuszak et al., "Detection of circulating tumor cells by means of machine learning using Smart-Seq2 sequencing," Scientific Reports, vol. 14, no. 1, p. 11057, 2024,http://dx.doi.org/10.1038/s41598-024.
[15] J. Ma et al., "Artificial intelligence based on blood biomarkers including CTCs predicts outcomes in epithelial ovarian cancer: A prospective study," OncoTargets and therapy, pp. 3267-3280, 2021,https://doi.org/10.2147/OTT.S307546.
[16] K. Sharifani and M. Amini, "Machine learning and deep learning: A review of methods and applications," World Information Technology and Engineering Journal, vol. 10, no. 07, pp. 3897-3904, 2023, https://papers.ssrn.com/sol3/ abs_id=4458723.
[17] M. M. Taye, "Understanding of machine learning with deep learning: architectures, workflow, applications and future directions," Computers, vol. 12, no. 5, p. 91, 2023,https://doi.org/10.3390/compu.
[19] R. Rejuan et al., "Validation of a Microfluidic Device Prototype for Cancer Detection and Identification: Circulating Tumor Cells Classification Based on Cell Trajectory Analysis Leveraging Cell-Based Modeling and Machine Learning," bioRxiv, 2024, https://doi.org/10.1002/cnm.70037.
[20] B. S. Abunasser, M. R. J. AL-Hiealy, I. S. Zaqout, and S. S. Abu-Naser, "Literature review of breast cancer detection using machine learning algorithms," in AIP Conference Proceedings, 2023, vol. 2808, no. 1: AIP Publishing, https://doi.org/10.1063/5.01336.
[21] B. Y. Kasula, "Machine Learning Applications in Diabetic Healthcare: A Comprehensive Analysis and Predictive Modeling," International Numeric Journal of Machine Learning and Robots, vol. 7, no. 7, 2023, https:// researchgate.net/Machine_Learning.
[22] E. K. Oikonomou and R. Khera, "Machine learning in precision diabetes care and cardiovascular risk prediction," Cardiovascular Diabetology, vol. 22, no. 1, p. 259, 2023,https://10.1186/s12933-023.
[23] S. N. A. Shah and R. Parveen, "An extensive review on lung cancer diagnosis using machine learning techniques on radiological data: state-of-the-art and perspectives," Archives of Computational Methods in Engineering, vol. 30, no. 8, pp. 4917-4930, 2023,https:// 10.1007/s11831-023.
[24] Q. Gao, L. Yang, M. Lu, R. Jin, H. Ye, and T. Ma, "The artificial intelligence and machine learning in lung cancer immunotherapy," Journal of Hematology & Oncology, vol. 16, no. 1, p. 55, 2023,https:// 10.1186/s13045-023-01456-y.
[25] L. Chen et al., "Machine Learning Predicts Oxaliplatin Benefit in Early Colon Cancer," Journal of Clinical Oncology, vol. 42, no. 13, pp. 1520-1530, 2024,https://doi.org/10.1200/JCO.23.01080.
[26] M. A. Islam, M. Z. H. Majumder, and M. A. Hussein, "Chronic kidney disease prediction based on machine learning algorithms," Journal of pathology informatics, vol. 14, p. 100189, 2023,https://doi.org/10.1016/j.jpi.2023.100189.
[27] S. F. Ahmed et al., "Deep learning modelling techniques: current progress, applications, advantages, and challenges," Artificial Intelligence Review, vol. 56, no. 11, pp. 13521-13617, 2023,https:// 10.1007/S10462-023-10466-8.
[28] C. Shen et al., "Automatic detection of circulating tumor cells and cancer associated fibroblasts using deep learning," Scientific reports, vol. 13, no. 1, p. 5708, 2023,https:// s41598-023-32955.
[29] Y. Kumar, R. Singh, M. R. Moudgil, and Kamini, "A systematic review of different categories of plant disease detection using deep learning-based approaches," Archives of Computational Methods in Engineering, vol. 30, no. 8, pp. 4757-4779, 2023,https://10.1007/s11831-023-09958-1.
[30] W. Duan and B. Ren, "Application Effects of NNN-link Care Model in Patients with Coronary Heart Disease," in The Heart Surgery Forum, 2023, vol. 26, no. 5, pp. E592-E599, https://doi.org/10.59958/hsf.58.
[31] A. K. Swain, A. Swetapadma, J. K. Rout, and B. K. Balabantaray, "A Review on Lung Cancer Detection and Classification using Shallow Learning and Deep Learning," in 2023 OITS International Conference on Information Technology (OCIT), 2023: IEEE, pp. 570-573, 10.1109/OCIT59427.2023.10431005.
[32] M. T. Ahad, Y. Li, B. Song, and T. Bhuiyan, "Comparison of CNN-based deep learning architectures for rice diseases classification," Artificial Intelligence in Agriculture, vol. 9, pp. 22-35, 2023,https://doi.org/10.1016/j.aiia.2023.07.001.
[33] G. E. Rao, B. Rajitha, P. N. Srinivasu, M. F. Ijaz, and M. Woźniak, "Hybrid framework for respiratory lung diseases detection based on classical CNN and quantum classifiers from chest X-rays," Biomedical Signal Processing and Control, vol. 88, p. 105567, 2024,https://doi.org/10.1016/j.bspc.2023.105567.
[34] S. Wang, Y. Zhou, X. Qin, S. Nair, X. Huang, and Y. Liu, "Label-free detection of rare circulating tumor cells by image analysis and machine learning," Scientific reports, vol. 10, no. 1, p. 12226, 2020,https:// s41598-020-69056.
[35] J. A. Pruneski et al., "Supervised machine learning and associated algorithms: applications in orthopedic surgery," Knee Surgery, Sports Traumatology, Arthroscopy, vol. 31, no. 4, pp. 1196-1202, 2023,https:// 10.1007/s00167-022.
[36] S. Naeem, A. Ali, S. Anam, and M. M. Ahmed, "An unsupervised machine learning algorithms: Comprehensive review," International Journal of Computing and Digital Systems, 2023,https://www.researchgate.net/_An_Unsupervised.
[37] D. Valkenborg, M. Geubbelmans, A.-J. Rousseau, and T. Burzykowski, "Supervised learning," American Journal of Orthodontics and Dentofacial Orthopedics, vol. 164, no. 1, pp. 146-149, 2023, https:// chapter/10.1007/978-3-540.
[38] S. Patil and S. Patil, "Linear with polynomial regression: Overview," Int J Appl Res, vol. 7, pp. 273-275, 2021,https:// Linear_with_Polynomail_Reg.
[39] J. Isabona, A. L. Imoize, and Y. Kim, "Machine learning-based boosted regression ensemble combined with hyperparameter tuning for optimal adaptive learning," Sensors, vol. 22, no. 10, p. 3776, 2022,https://doi.org/10.3390/s22103776.
[40] E. Hatzidaki, A. Iliopoulos, and I. Papasotiriou, "A novel method for colorectal cancer screening based on circulating tumor cells and machine learning," Entropy, vol. 23, no. 10, p. 1248, 2021,https://doi.org/10.3390/s22103776.
[41] H. Li et al., "Digital Quantitative Detection for Heterogeneous Protein and mRNA Expression Patterns in Circulating Tumor Cells," Advanced Science, p. 2410120, 2025, https://doi.org/10.1002.
[42] P. Chen et al., "Detection of circulating plasma cells in peripheral blood using deep learning‐based morphological analysis," Cancer, vol. 130, no. 10, pp. 1884-1893, 2024,https://doi.org/10.1002/cncr.352.
[43] L. Gerratana et al., "Integrating machine learning-predicted circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) in metastatic breast cancer: A proof of principle study on endocrine resistance profiling," Cancer Letters, vol. 609, p. 217325, 2025,https://doi.org/10.1016/j.canlet.2024.
[44] T. M. Scholtens, F. Schreuder, S. T. Ligthart, J. F. Swennenhuis, J. Greve, and L. W. Terstappen, "Automated identification of circulating tumor cells by image cytometry," Cytometry Part A, vol. 81, no. 2, pp. 138-148, 2012, https://doi.org/10.1002/cyto.a.
[45] I. Mocan, R. Itu, A. Ciurte, R. Danescu, and R. Buiga, "Automatic Detection of Tumor Cells in Microscopic Images of Unstained Blood using Convolutional Neural Networks," in 2018 IEEE 14th International Conference on Intelligent Computer Communication and Processing (ICCP), 2018: IEEE, pp. 319-324, 10.1109/ICCP.2018.8516638.
[46] A. Ciurte, C. Selicean, O. Soritau, and R. Buiga, "Automatic detection of circulating tumor cells in darkfield microscopic images of unstained blood using boosting techniques," PloS one, vol. 13, no. 12, p. e0208385, 2018,https://doi.org/10.1371/journal.
[47] L. L. Zeune et al., "Deep learning of circulating tumour cells," Nature Machine Intelligence, vol. 2, no. 2, pp. 124-133, 2020,https:// s42256-020-0153.
[48] L. Zeune et al., "Quantifying HER-2 expression on circulating tumor cells by ACCEPT," PloS one, vol. 12, no. 10, p. e0186562, 2017,https://doi.org/10.1371/journal.pone.0186562.
[49] B. He et al., "A new method for CTC images recognition based on machine learning," Frontiers in Bioengineering and Biotechnology, vol. 8, p. 897, 2020, https://www.frontiersin.org/journals/bioeng.
[50] Z. Guo, X. Lin, Y. Hui, J. Wang, Q. Zhang, and F. Kong, "Circulating tumor cell identification based on deep learning," Frontiers in Oncology, vol. 12, p. 843879, 2022, https:// front.org/journals/oncology.
مقدس,هاجر و پاکباز,حمید . (1404). کاربرد هوش مصنوعی در تشخیص سلول سرطانی در جریان خون. مجله مهندسی مکانیک, 34(5), 19-27. doi: 10.30506/mmep.2025.2065660.2249
MLA
مقدس,هاجر , و پاکباز,حمید . "کاربرد هوش مصنوعی در تشخیص سلول سرطانی در جریان خون", مجله مهندسی مکانیک, 34, 5, 1404, 19-27. doi: 10.30506/mmep.2025.2065660.2249
HARVARD
مقدس هاجر, پاکباز حمید. (1404). 'کاربرد هوش مصنوعی در تشخیص سلول سرطانی در جریان خون', مجله مهندسی مکانیک, 34(5), pp. 19-27. doi: 10.30506/mmep.2025.2065660.2249
CHICAGO
هاجر مقدس و حمید پاکباز, "کاربرد هوش مصنوعی در تشخیص سلول سرطانی در جریان خون," مجله مهندسی مکانیک, 34 5 (1404): 19-27, doi: 10.30506/mmep.2025.2065660.2249
VANCOUVER
مقدس هاجر, پاکباز حمید. کاربرد هوش مصنوعی در تشخیص سلول سرطانی در جریان خون. MMEP, 1404; 34(5): 19-27. doi: 10.30506/mmep.2025.2065660.2249